Proteomics profiling of Madin-Darby canine kidney plasma membranes reveals Wnt-5a involvement during oncogenic H-Ras/TGF-β-mediated epithelial-mesenchymal transition

YS Chen; RA Mathias; S Mathivanan; EA Kapp; RL Moritz; HJ Zhu; RJ Simpson

Journal article

Proteomics profiling of Madin-Darby canine kidney plasma membranes reveals Wnt-5a involvement during oncogenic H-Ras/TGF-β-mediated epithelial-mesenchymal transition

YS Chen, RA Mathias, S Mathivanan, EA Kapp, RL Moritz, HJ Zhu, RJ Simpson

Molecular and Cellular Proteomics | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2011

DOI: 10.1074/mcp.M110.001131

Abstract

Epithelial-mesenchymal transition (EMT) describes a process whereby polarized epithelial cells with restricted migration transform into elongated spindle-shaped mesenchymal cells with enhanced motility and invasiveness. Although there are some molecular markers for this process, including the down-regulation of E-cadherin, our understanding of plasma membrane (PM) and associated proteins involved in EMT is limited. To specifically explore molecular alterations occurring at the PM, we used the cationic colloidal silica isolation technique to purify PM fractions from epithelial Madin-Darby canine kidney cells during Ras/TGF-β-mediated EMT. Proteins in the isolated membrane fractions were separ..

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University of Melbourne Researchers

Hongjian Zhu Author

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Grants

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This work was supported by National Health and Medical Research Council of Australia Program Grant 487922 (to R.J.S.) and Grants 280913 and 433619 (to H.-J.Z.), The Australian Government Endeavor International Postgraduate Research Scholarship and The University of Melbourne International Research Scholarship (to Y.-S.C.), and The University of Melbourne Research Scholarship (to R.A.M.).